A simple STAR Element Repetitive-PCR (SER-PCR) typing method for the rapid characterization of Staphylococcus aureus

نویسندگان

چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Clinical evaluation of the DiversiLab microbial typing system using repetitive-sequence-based PCR for characterization of Staphylococcus aureus strains.

The DiversiLab System, which includes microfluidics-based detection, reagent kits, and software for data processing and analysis, is an automated method using repetitive sequence-based PCR (rep-PCR) for microbial strain typing. To assess the reliability of the DiversiLab System for strain characterization of Staphylococcus aureus, we tested clinical isolates sent to ARUP Laboratories for typing...

متن کامل

A rapid, simple method for detecting PCR failure.

Department of Medical Microbiology, St. Bartholomew's Hospital Medical College, London, EC1A 7BE, UK The polymerase chain reaction (PCR) holds great potential for the rapid and sensitive detection of pathogenic microorganisms. (1~ There has been much interest in detecting and preventing falsepositive PCR results owing to product carry-over(2); however, less attention has been paid to false-nega...

متن کامل

A rapid and simple method for reversing the inhibitory effect of heparin on PCR for HLA class II typing.

Centro Trasfusionale e di Immunologia dei Trapianti, Ospedale Maggiore Policlinico, 20122 Milan, Italy Blood samples collected with heparin as anticoagulant have been shown to yield decreased quantities of DNA, and an inhibitory effect of this anticoagulant on PCR has been demonstrated. Furthermore, in our experience, the amplification by PCR of HLA-DRB1 second exon was unsuccessful when the DN...

متن کامل

Multicenter evaluation of arbitrarily primed PCR for typing of Staphylococcus aureus strains.

Fifty-nine isolates of Staphylococcus aureus and a single strain of Staphylococcus intermedius were typed by arbitrarily primed PCR (AP-PCR). To study reproducibility and discriminatory abilities, AP-PCR was carried out in seven laboratories with a standardized amplification protocol, template DNA isolated in a single institution, and a common set of three primers with different resolving power...

متن کامل

Multiplex PCR strategy for rapid identification of structural types and variants of the mec element in methicillin-resistant Staphylococcus aureus.

Full characterization of methicillin-resistant Staphylococcus aureus (MRSA) requires definition of not only the bacterial genetic background but also the structure of the complex and heterologous mec element these bacteria carry, which is associated with drug resistance determinant mecA. We report the development, validation, and application of a multiplex PCR strategy that allows quick presump...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: The Journal of Infection in Developing Countries

سال: 2019

ISSN: 1972-2680

DOI: 10.3855/jidc.11404